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KMID : 0903619980390030312
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Journal of the Korean Society for Horticultural Science
1998 Volume.39 No. 3 p.312 ~ p.315
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PCR Amplification of a β-Galactosidase cDNA Clone from Persimmon Fruit
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강인규/Kang, In Kyu
변재균/서상곤/Kenneth, C . Gross/Byun, Jae Kyun/Suh, Sang Gon
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Abstract
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This study was carried out to amplify and clone of persimmon β-galactosidase cDNA based on a consensus N-terminal amino acid sequence of apple, asparagus, persimmon and carnation β-galactosidase by polymerise chain reaction (PCR). PCR amplified β-galactosidase was a DNA fragment of 849 bp. It was cloned into the pCR-Script cloning vector (pPBG8). The N-terminal sequence of the 44-kDa protein showed strong homology with other recently identified β-galactosidase from tomato, apple, asparagus and carnation. The N-terminal amino acid sequence of the 44-kDa protein showed 60% identity with those of β-galactosidase amino acid sequence from asparagus, 70% identity with apple, 45% identity with carnation and 45% identity with tomato. The deduced amino acid sequence of pPBG8 cDNA showed 100% identity with the N-terminal amino acid sequence (11-Val to 20-Ile) from persimmon fruit. The deduced amino acid sequence of pPBGB cDNA showed 75% identity with those of β-galactosidase clone from asparagus, 72% identity with apple, 66% identity with carnation and 73% identity with tomato. pPBG8 showed a greater deviation from the consensus sequence of the other 4 clones which showed a higher degree of homology toward each other.
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